Tuğçe Haskavuk

• Improving the HPV diagnosis kit and biosensors
• DNA isolation, PCR, and agarose gel electrophoresis
• Prepared comprehensive reports summarizing data analysis findings, informing key decision-makers of important trends.
• Prepared project presentations and reports to assist senior staff.

• Article publishing
• Knowledge of culturing, subculturing, and maintaining various cell lines, including primary and immortalized cells.
• Proficiency in using different types of microscopes (light, fluorescence, electron) to observe and analyze cell morphology, growth, and behavior.
• Ability to perform cell counting using hemocytometers or automated cell counters.
• Skills in preparing and optimizing culture media, including the addition of growth factors, supplements, and antibiotics.
• Techniques for freezing and thawing cells for long-term storage without compromising viability.
• Ability for cell passaging .
• Skill in handling and manipulating chicken embryos, including careful opening of eggs and maintaining sterile conditions.
• Expertise in preparing the chorioallantoic membrane for the assay, which includes windowing the eggshell and properly exposing the membrane without damaging it.
• Mastery of aseptic techniques to avoid contamination during the CAM assay setup and throughout the experiment.
• Ability to assess and quantify angiogenesis, which involves evaluating the growth of blood vessels in response to the applied samples.
• Proficiency in using imaging equipment as light microscopy and fluorescence microscopy, to capture high-quality images of the CAM and its vasculature.
• Understanding of ethical considerations and regulations regarding the use of animal embryos in research.

This training focused on separation cells based on physical, chemical, and mechanical properties in centrifugal microfluidics, with potential applications in cancer cell analysis, point-of-care tests, and biomedical device development.

• Using electron microscope.
• Preparing microfluidic chips with PDSM.
• Working with centrifugal microfluidic chips.
• Spin-controlled centrifuge operation made for handling cells, particles, and homogeneous liquids.
• Studying buffy coat formations, counting white blood cells, and separating beads by size and density using wafer and blood centrifugation chips by using wafer chips and PDMS chips.
• Isopycnic separation with Ficoll to separate beads by density.
• Agarose gel electrophoresis testing using blood centrifugation chips.

• Working in the R&D department as an R&D specialist
• Sequencing by using Oxford Nanopore Technologies, and carrying out the work of our brand, Massbiome, which is based on microbiome analysis in the human gut
• Managing R&D studies for company, and improving the sepsis disease detection kit
• Coordinating the routine work of the laboratories and train interns
• Experienced with PCR, qPCR, library preparation for DNA sequencing with Oxford Nanopore Technologies, and DNA/RNA extraction from different samples with different methods.
• Promoted a safe working environment by adhering strictly to safety regulations during laboratory procedures.
• Enhanced product development by conducting thorough research and devising innovative strategies.
• Presented research findings at conferences, showcasing the company''s expertise and innovative approach to problem solving.

• Website: https://www.oulu.fi/en
• I worked on the vascularization of Kidney organoids on a vascular bed and microfluidic chip production with PDMS. I did a CAM assay using chicken eggs. I worked with human cell cultures. I used an electron microscope, and I did cell counting. Our article is currently in the process of being published.

• Final Grade: 3.21
• Website: https://iyte.edu.tr/

• Final Grade: 98.07