
An accomplished and driven scientist with nearly fifteen years of expertise in cellular and molecular biology. Demonstrates adaptability across diverse subjects within the educational realm and current professional scope. Proficient in various molecular techniques, particularly conducting cell-based experiments, employing multiple PCR techniques, and conducting NGS studies. Collaborative and encouraging in joint research projects, displaying the ability to successfully fulfill responsibilities. Equally effective as a team player or working independently.
• Amplicon and enrichment based NGS panel development
• Illumina Systems applications such as ISeq and MiniSeq, BioRad CFX and ddPCR, and Opus applications.
• R&D Screening and Diagnostic Kit development, including
RefKit Spinal Muscular Atrophy qPCR Screening Kit - SMN1 (https://refgen.com/en/refkit-spinalmuscular-atrophy-qpcrscreening-kit-smn1/),
RefKit Spinal Muscular Atrophy qPCR Screening Kit SMN1/SMN2 (https://refgen.com/en/refkit-spinalmuscularatrophy-qpcr-screening-kit-smn1-smn2/), and
RefPlex Spinal Muscular Atrophy NGS Kit.
Routine tasks in the kit development process include keeping records of experiments carried out, studying and validating products developed on different devices, taking witness samples for each product and studying them at certain intervals, and reporting all necessary information on the process to the quality control department.
PCR ( qRT-PCR, Multiplex PCR, OPUS, ddPCR)
Next Generation Sequencing ( Amplicon based of Enrichment (hybridization) based library preparations, ISeq, MiniSeq Applications)
Sequencing analysis ( Galaxy, GenomeBrowse)
Use of R Studio ( Script writing for NGS analysis and visualization of results)
Data analysis and interpretation
Mammalian cell culture (Transfection techniques, Monoclonal selection, Phenotypic assays, DNA/RNA extractions, RNA interference)
Cell based assays (Luciferase)
Bacterial culture techniques (cloning, transformation, extractions)
Protein based techniques (Nuclear/cytoplasmic isolations, Western Blotting, IP, Flow cytometry)
Troubleshooting
Keeping record and reporting experimental details
Effective time management and planned schedule
Supportive teammate
Work effectively and reproductively alone
Project management